Access to Biobank
Estonian Biobank is a population-based biobank of the Estonian Genome Center at the University of Tartu (EstBB).
The entire project is conducted according to the legislation given in the Human Genes Research Act of Estonia and all participants (gene donors) have signed the broad inform consent (Metspalu 2004, Drug Dev Res).
The Participants have filled the Computer Assisted Personal Interview (CAPI) within 1-2 hours appointment at a doctor’s office.
The CAPI includes personal data (place of birth, place(s) of living, nationality etc.), genealogical data (family history of medical conditions spanning four generations), educational and occupational history, lifestyle data (physical activity, dietary habits - FFQ, smoking, alcohol consumption, women’s health, quality of life).
In addition, there are 40,000 participants with MCTQ (chronotype) data, and 15,000 with both MSTQ and genome-wide microarray (GWAS) data, 3,000 participants have filled the NEO-PI-R questionnaire, including GWAS data available on 2,700 participants.
Medical history and current health status is recorded according to ICD-10 codes, medication according to ATC. Extra data are collected from patients with psychiatric disorders (MINI and SSP interview). Anthropometric measurements, blood pressure (sitting position at the end of the interview), and resting heart rate are measured during the visit; 30-50 ml of venous blood are taken into EDTA Vacutainers, which are transported to the central laboratory of EstBB, being kept at +4°...+6°C within the following 6 to 36 hrs, where DNA, plasma and WBC are immediately isolated and stored as aliquots in MAPI straws in liquid N2 for further use. All procedures have been set up according to the ISO 9000-2015 (active sertificate 1.09.2003-09.09.2020) and LIMS procedures. Currently we are preparing for the sertification process for ISO 17025:2017 sertificate.
GWAS analyses have been performed on 15,000 subjects genotyped with different Illumina microarrays. RNA samples from 2,100 subjects (isolated from venous blood) are available for gene expression studies along with 45 biomarkers from serum and plasma (analysis of biomarkers was performed in Clinical chemistry laboratory at Tartu University Hospital. Metabolite analysis (NMR and MS) from the same set of individuals (N=12,000 in total) will be performed with the ENGAGE consortium during the final stage. To date, 50,000 biobank participants have been genotyped with the Global Screening Array from Illumina. An additional 100,000 participants will be recruited and genotyped 2018-2019 In addition, we link our database with the national registries and hospital databases in order to get the up- to-date phenotypic information (endpoints and nonfatal events).
The publications of the EstBB
The EstBB publishing close to 100 publcations every year, including high impact journals as Nature, Nature Genetics, AJHG etc. More information in available at www.biobank.ee
The team of the EstBB
There are a team of 51 people working at the research unit and the biobank. The research unit is divided into three workgroups: biostatistics, bioinformatics and functional genomics. The support stucture consists of an IT unit and administrative staff.