09.09.2026

Next Generation Sequencing

The University of Tartu Core Facility of Genomics delivers high-quality long-read and short-read sequencing services, supporting applications from targeted analyses to population-scale genomics research. We help researchers identify the most effective sequencing approach for their scientific objectives and provide end-to-end support through advanced laboratory workflows and integrated bioinformatics expertise.

Why work with us?

Comprehensive sequencing expertise
We support diverse genomics applications using PacBio, Oxford Nanopore, and Illumina technologies. Our experience includes one of Europe's largest long-read population genomics initiatives, involving whole-genome sequencing of 10,000 Estonian Biobank participants.

Read more: https://genomics.ut.ee/en/news/new-technology-help-sequence-whole-genom…

One integrated team from start to finish
Laboratory scientists and bioinformaticians collaborate closely to ensure seamless communication and efficient project delivery. From study design and sample preparation to sequencing, data processing, and interpretation, we provide coordinated support tailored to your research goals.

Sequencing applications

1. Long-read sequencing
Long-read sequencing is particularly valuable for applications requiring comprehensive genome analysis, structural variant detection and direct DNA methylation profiling.

Typical applications

  • Human whole genome sequencing
  • Structural variant analysis
  • DNA methylation analysis
  • Metagenomics
  • Amplicon sequencing
  • Plasmid sequencing

2. Short-read sequencing
Short-read sequencing provides robust, high-throughput solutions for established genomics workflows.

Typical applications

  • Whole exome sequencing
  • RNA sequencing
  • Small genome sequencing
  • Metagenomics
  • Amplicon sequencing
  • Gene panel sequencing

Sequencing platforms and sample quality control

PacBio HiFi sequencing combines long read lengths with high accuracy for whole genome sequencing, DNA methylation profiling and accurate analysis of complex genomic regions.

Applications

  • Human whole genome sequencing
  • DNA methylation analysis (5mC and 6mA)
  • Metagenomics
  • Amplicon sequencing
  • Plasmid sequencing
PacBio_Revios
Author: Genoomika instituut

Ultra-long read sequencing for comprehensive genome analysis and epigenetic profiling.

Applications

  • Whole genome sequencing
  • Metagenomics
  • Amplicon sequencing
  • Plasmid sequencing
  • RNA sequencing
  • DNA methylation analysis (5mC, 5hmC, 6mA and 4mC)

ONT P2 Solo
Author: Genoomika instituut

High-throughput short-read sequencing for established genomics workflows.

Applications

  • Whole exome sequencing
  • Small genome sequencing
  • RNA sequencing
  • Metagenomics
  • Amplicon and gene panel sequencing

Illumina NextSeq 2000 System

NextSeq2000
Author: Genoomika instituut

Illumina MiSeq System

MiSeq
Author: Genoomika instituut

High-quality sequencing begins with high-quality input material. We provide DNA and RNA quality assessment, fragment size analysis, quantification and sample preparation to support successful sequencing projects across all sequencing platforms.

Available technologies

  • Agilent TapeStation 4200
  • Agilent Femto Pulse
  • Covaris M220
  • Yourgene Health LightBench Detect
  • Applied Biosystems real-time PCR system
  • Initial data quality control and export in a preferred format (.vcf)
  • RNA‑seq and exome analysis, human whole‑genome analysis

To discuss your project and specific pricing please contact [email protected].