Sanger sequencing and genotyping

Sequencing full service

For ordering full sequencing You'll have to provide us with:

• Template DNA - 300-500 ng of plasmid per reaction, 30-50 ng PCR product per reaction. We can use up to 5,7 μL of template DNA in each reaction mixture
• Primers - for one sequencing reaction we require 0,3 μL of 10 µM (10 pmol/mL) primer (but please send no less than 2-3 μL)
• Complete the order form and send it to [email protected];
  • print it out as well and send it with DNA and primers to the address:
    Institute of Genomics Core Facility
    University of Tartu
    Riia mnt 23b-302
    51010 Tartu, ESTONIA
  • Or bring your samples to the freezer by the security table at our building's Riia street entrance.

Sample submission instructions:

DNA samples should be submitted in 200 μL 8-well strip tubes with individually attached frosted caps or half-skirted PCR plates.

Primers should be submitted in 1,5 mL nuclease-free tubes or 200 μL 8-well strip tubes.

For orders with more than seven samples in separate 200 μL tubes, a handling fee of up to 50% may apply.

We recommend using the following or equivalent 8-well strips:

• Eppendorf PCR Tube Strips, product number 0030124359
• Nerbe Plus PCR Microcentrifuge Tube PP, product number 04-032-0600

Label the sample tubes with numbers starting with 1 or another short identifier. The sample label must match the "Label on tube" column on the order form. If multiple reactions need to be performed with one sample, repeat the label for the same sample on the form. The names of the result files are formed from the "Sample name" and "Primer name" columns.

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If you are using a plate, please fill it according to the columns in A01, B01, C01, etc order. The plate marking must be on the side of the plate, not on the cover.

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We offer the following universal primers: