We offer the following universal primers:
Primer | Sequence | length (bp) |
ITS4 | TCCTCCGCTTATTGATATGC | 20 |
ITS5 | GGAAGTAAAAGTCGTAACAAGG | 22 |
M13F | GTAAAACGACGGCCAGT | 17 |
M13F-pUC(-40) | GTTTTCCCAGTCACGAC | 17 |
M13R | GCGGATAACAATTTCACACAGG | 22 |
M13R-pUC(-40) | CAGGAAACAGCTATGAC | 17 |
pJET1.2F | CGACTCACTATAGGGAGAGCGGC | 23 |
pJET1.2R | AAGAACATCGATTTTCCATGGCAG | 24 |
SP6 | ATTTAGGTGACACTATAG | 18 |
T3 | ATTAACCCTCACTAAAG | 17 |
T7 | AATACGACTCACTATAG | 17 |
T7 promoter | TAATACGACTCACTATAGGG | 20 |
T7 terminator | GCTAGTTATTGCTCAGCGG | 19 |
We also analyze ready-to-load sequencing reaction products. Products must be manufactured using the BigDye® Terminator v3.1 (in exceptional cases You can also use BigDye® Terminator v1.1) and diluted in 10 μL formamide (70% formamide or Applied Biosystems Hi-Di formamide).
There is no need to fill the order form, please send us the list of sample names in *.txt file, along with invoice recipient.
We use a variety of fragment analysis methods - microsatellite analysis, MLPA analysis etc. The client generally prepares the samples to a ready-to-load state, we perform the denaturation before analysis. There is no need to fill the order form, please send us the list of sample names in *.txt file, along with invoice recipient and used dye set.
You can use following dye combinations:
Filter | Dye set | Blue | Green | Yellow | Red | Size standard |
D | DS-30 | 6FAM | HEX | NED | ROX | |
G5 | DS-33 | 6FAM | VIC | NED | PET | LIZ |
E5 | DS-02 | dR110 | dR6G | dTAMRA | dROX | LIZ |
4Dye | Custom | 6FAM | HEX | TAMRA | ROX | |
5Dye | Custom | FL | JOE | TMR-ET | CXR-ET | WEN |
F | Promega | FL | JOE | TMR | CXR |
Note that the size standard is added to each sample. For best quality results be sure to use Applied Biosystems Hi-Di Formamide as a loading solution (minimum 10 μL).
The batch size for fragment analysis is 24 samples.
With a fast processing service, we guarantee the results by the next working day. Note that the samples must arrive by 9 am if You want to receive results within the same working day. Fast processing service by the same day is not available on public shortened working days.
For ordering a fast processing service, please write it to the "Comments" section in the order form.